Hotspot

bas1-rpl35

Genome · 19216-19951 · 736 bp

Back to Kumanoa

Pi 0.302

MarkerSeek score 73.60

Primer available yes

Label bas1-rpl35

Feature Metadata

label_name: bas1-rpl35
feature_type: igs
region: Genome
length_bp: 736
variable_sites: 127
pi: 0.302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.57
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
bas1-rpl35_p1	CAGACAGAACGGGAGAATGG	CAACAGCTATTTGGGCAAGC	55.0	50.0	58.0	58.0	1634 bp	0.5	59.9
bas1-rpl35_p2	GCAGCCAGGAGATAAGACAC	CAACAGCTATTTGGGCAAGC	55.0	50.0	58.1	58.0	1368 bp	0.5	59.6
bas1-rpl35_p3	AGACAGAACGGGAGAATGGA	CAACAGCTATTTGGGCAAGC	50.0	50.0	58.1	58.0	1633 bp	0.5	59.5
bas1-rpl35_p4	CAGACAGAACGGGAGAATGG	ACAGCTATTTGGGCAAGCAT	55.0	45.0	58.0	58.1	1632 bp	0.5	58.7
bas1-rpl35_p5	AGAACGGGAGAATGGAGGAT	CAACAGCTATTTGGGCAAGC	50.0	50.0	57.8	58.0	1629 bp	0.5	58.5