Hotspot

infA

LSC · 74736-74972 · 237 bp

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Pi 0.0378

MarkerSeek score 70.70

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 237
variable_sites: 13
pi: 0.0378
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	TGATTCCCTTTCCCACGAAA	TCTCATAGACAAAATGTATCCGA	45.0	34.8	57.0	55.0	298-392 bp	0.666667	31.3
infA_p2	TTGATTCCCTTTCCCACGAA	TCTCATAGACAAAATGTATCCGA	45.0	34.8	57.0	55.0	299-393 bp	0.666667	31.3
infA_p3	ATTGATTCCCTTTCCCACGA	TCTCATAGACAAAATGTATCCGA	45.0	34.8	56.8	55.0	300-394 bp	0.666667	31.3
infA_p4	TTGATTCCCTTTCCCACGAAA	TCTCATAGACAAAATGTATCCGA	42.9	34.8	57.8	55.0	299-393 bp	0.666667	31.3
infA_p5	TGATTCCCTTTCCCACGAAA	TCTCATAGACAAAATGTATCCGAA	45.0	33.3	57.0	55.7	298-392 bp	0.666667	31.3