Hotspot

trnN-GUU-trnR-ACG

IRa · 120292-120567 · 276 bp

Back to Kuepferia

Pi 0.305

MarkerSeek score 79.50

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 276
variable_sites: 105
pi: 0.305
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	296-474 bp	1.0	89.0
trnN-GUU-trnR-ACG_p2	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	300-478 bp	1.0	88.1
trnN-GUU-trnR-ACG_p3	GGTTCGAATCCTACTTGGGG	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.7	57.8	288-466 bp	1.0	87.7
trnN-GUU-trnR-ACG_p4	TTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.3	57.8	297-475 bp	1.0	85.0
trnN-GUU-trnR-ACG_p5	TGGTCGTAGGTTCGAATCCT	GGCCTGTAGCTCAGAGGATT	50.0	55.0	58.2	58.9	307-485 bp	1.0	83.8