Hotspot

ndhG-ndhI

SSC · 110127-110516 · 390 bp

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Pi 0.0364

MarkerSeek score 70.20

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 390
variable_sites: 20
pi: 0.0364
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ACCAATCCCAAGGAAAAAGCA	ACCGACATCAGTAATTGAGGA	42.9	42.9	58.3	56.5	470-495 bp	1.0	56.5
ndhG-ndhI_p2	CCAATCCCAAGGAAAAAGCAG	ACCGACATCAGTAATTGAGGA	47.6	42.9	57.7	56.5	469-494 bp	1.0	56.4
ndhG-ndhI_p3	ACCAATCCCAAGGAAAAAGCA	TCAAATTGCTTTAGGTCGGT	42.9	40.0	58.3	55.3	491-516 bp	1.0	54.8
ndhG-ndhI_p4	CCAATCCCAAGGAAAAAGCA	ACCGACATCAGTAATTGAGGA	45.0	42.9	56.5	56.5	469-494 bp	1.0	54.8
ndhG-ndhI_p5	CCAATCCCAAGGAAAAAGCAG	TCAAATTGCTTTAGGTCGGT	47.6	40.0	57.7	55.3	490-515 bp	1.0	54.7