Hotspot

rpl32-trnL-UAG

SSC · 105317-105909 · 593 bp

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Pi 0.0411

MarkerSeek score 74.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 593
variable_sites: 35
pi: 0.0411
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAAGGGATATTGGACCGCTT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.4	57.9	676-738 bp	1.0	82.7
rpl32-trnL-UAG_p2	GAAGGGATATTGGACCGCTT	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.4	57.8	669-731 bp	1.0	82.3
rpl32-trnL-UAG_p3	GAAGGGATATTGGACCGCTT	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.4	57.8	675-737 bp	1.0	82.1
rpl32-trnL-UAG_p4	GAAGGGATATTGGACCGCTT	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.4	57.8	674-736 bp	1.0	82.1
rpl32-trnL-UAG_p5	GAAGGGATATTGGACCGCTT	AAGATGACATGCCGCTACTC	50.0	50.0	57.4	57.8	726-788 bp	1.0	81.5