Hotspot

petL-petG

LSC · 61489-61657 · 169 bp

Back to Kuepferia

Pi 0.0375

MarkerSeek score 71.50

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 9
pi: 0.0375
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	CGATAACCTCCGCTCTGTTT	GCAGTGACGAATAATCCAGC	50.0	50.0	57.7	57.0	289-300 bp	1.0	74.9
petL-petG_p2	ACTCGCGGCTTTAACGATAA	GCAGTGACGAATAATCCAGC	45.0	50.0	57.7	57.0	303-314 bp	1.0	74.7
petL-petG_p3	ACCTCCGCTCTGTTTATTGG	GCAGTGACGAATAATCCAGC	50.0	50.0	57.6	57.0	284-295 bp	1.0	74.1
petL-petG_p4	CCTCCGCTCTGTTTATTGGT	GCAGTGACGAATAATCCAGC	50.0	50.0	57.6	57.0	283-294 bp	1.0	74.1
petL-petG_p5	CTCGCGGCTTTAACGATAAC	GCAGTGACGAATAATCCAGC	50.0	50.0	57.4	57.0	302-313 bp	1.0	72.0