Hotspot

psbL-psbF

LSC · 60180-60202 · 23 bp

Back to Kuepferia

Pi 0.058

MarkerSeek score 76.70

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 23
variable_sites: 2
pi: 0.058
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	CGTTTGGGTTTGATTGTGTCA	ACGGTCTAGCTGTACCTACC	42.9	55.0	57.6	58.0	113 bp	1.0	69.6
psbL-psbF_p2	CGTTTGGGTTTGATTGTGTCA	GGTCTAGCTGTACCTACCGT	42.9	55.0	57.6	58.0	111 bp	1.0	69.6
psbL-psbF_p3	CGTTTGGGTTTGATTGTGTCA	TTCACGGTCTAGCTGTACCT	42.9	50.0	57.6	57.8	116 bp	1.0	68.3
psbL-psbF_p4	CGTTTGGGTTTGATTGTGTCA	GTTCACGGTCTAGCTGTACC	42.9	55.0	57.6	57.7	117 bp	1.0	67.7
psbL-psbF_p5	TCGTTTGGGTTTGATTGTGTC	GGTCTAGCTGTACCTACCGT	42.9	55.0	57.3	58.0	112 bp	1.0	67.4