Hotspot

rbcL-accD

LSC · 52923-53612 · 690 bp

Back to Kuepferia

Pi 0.0856

MarkerSeek score 80.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 71
pi: 0.0856
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CTGTACTACAGTTCGGTGGC	GTACCGGGATCGATCGAAAG	55.0	55.0	58.0	58.0	1560-1633 bp	1.0	87.8
rbcL-accD_p2	CGTAGCGAATCGAGTAGCTC	GTACCGGGATCGATCGAAAG	55.0	55.0	58.1	58.0	1502-1575 bp	1.0	87.6
rbcL-accD_p3	GGGTCTCTCTACCAGGTGTT	GTACCGGGATCGATCGAAAG	55.0	55.0	58.1	58.0	1650-1723 bp	1.0	87.3
rbcL-accD_p4	TTGGGTCTCTCTACCAGGTG	GTACCGGGATCGATCGAAAG	55.0	55.0	58.1	58.0	1652-1725 bp	1.0	87.2
rbcL-accD_p5	CGGCCTACTTCTTCACATCC	GTACCGGGATCGATCGAAAG	55.0	55.0	58.1	58.0	1892-1965 bp	1.0	86.8