Hotspot

trnK-UUU-trnQ-UUG

LSC · 4208-5776 · 1569 bp

Back to Kuepferia

Pi 0.0515

MarkerSeek score 64.10

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1569
variable_sites: 49
pi: 0.0515
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.43
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	CGAATCCCTTGCTTCATCCA	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	1220-2237 bp	1.0	86.3
trnK-UUU-trnQ-UUG_p2	CGAATCCCTTGCTTCATCCA	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1225-2242 bp	1.0	86.3
trnK-UUU-trnQ-UUG_p3	ACGAATCCCTTGCTTCATCC	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	1221-2238 bp	1.0	86.3
trnK-UUU-trnQ-UUG_p4	GGACGAATCCCTTGCTTCAT	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	1223-2240 bp	1.0	86.3
trnK-UUU-trnQ-UUG_p5	ATGGACGAATCCCTTGCTTC	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	1225-2242 bp	1.0	86.2