Hotspot

ycf1

SSC · 122910-129644 · 6735 bp

Back to Krascheninnikovia

Pi 0.0086

MarkerSeek score 60.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 6735
variable_sites: 56
pi: 0.0086
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCCGTGGTATCATCGGTAT	GGACCCTTTCGAACAAGAGG	50.0	55.0	58.0	58.2	394-484 bp	1.0	84.3
ycf1_p2	TATCAAAGTCACTTCGCCCG	GGACCCTTTCGAACAAGAGG	50.0	55.0	58.0	58.2	427-517 bp	1.0	84.1
ycf1_p3	ACCCGTGGTATCATCGGTAT	ACTGGACCCTTTCGAACAAG	50.0	50.0	58.0	57.7	397-487 bp	1.0	83.7
ycf1_p4	TATCAAAGTCACTTCGCCCG	ACTGGACCCTTTCGAACAAG	50.0	50.0	58.0	57.7	430-520 bp	1.0	83.6
ycf1_p5	AAATCTTCGAACCACTCCGG	GGACCCTTTCGAACAAGAGG	50.0	55.0	57.9	58.2	891-981 bp	1.0	82.6