Hotspot

rbcL

LSC · 55586-57013 · 1428 bp

Back to Krascheninnikovia

Pi 0.0035

MarkerSeek score 60.80

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 5
pi: 0.0035
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGAAGTTGTAGGGAGGGACTT	AGTAAAAGATTGGGCCGAGT	47.6	45.0	58.0	56.8	1512 bp	1.0	62.8
rbcL_p2	TGAAGTTGTAGGGAGGGACTT	AGTAAAAGATTGGGCCGAGTT	47.6	42.9	58.0	57.6	1512 bp	1.0	60.7
rbcL_p3	TTGAAGTTGTAGGGAGGGAC	AGTAAAAGATTGGGCCGAGT	50.0	45.0	56.5	56.8	1513 bp	1.0	58.6
rbcL_p4	TTGAAGTTGTAGGGAGGGAC	AGTAAAAGATTGGGCCGAGTT	50.0	42.9	56.5	57.6	1513 bp	1.0	56.5
rbcL_p5	TGAAGTTGTAGGGAGGGACTT	GTAAAAGATTGGGCCGAGTT	47.6	45.0	58.0	56.0	1511 bp	1.0	56.4