Hotspot

rpl2-trnI-CAU

IRb · 85795-86603 · 809 bp

Back to Krascheninnikovia

Pi 0

MarkerSeek score 60.30

Primer available yes

Label rpl2-trnI-CAU

Feature Metadata

label_name: rpl2-trnI-CAU
feature_type: igs
region: IRb
length_bp: 809
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnI-CAU_p1	CAACGACGCTGTCCATAGAT	GAATGGTTAAAGCGCCCAAC	50.0	50.0	57.8	58.0	981-995 bp	1.0	79.3
rpl2-trnI-CAU_p2	CGTGTGCTCGAGTTAGAAGT	GAATGGTTAAAGCGCCCAAC	50.0	50.0	57.7	58.0	912-926 bp	1.0	78.4
rpl2-trnI-CAU_p3	CGACGCTGTCCATAGATCAA	GAATGGTTAAAGCGCCCAAC	50.0	50.0	57.5	58.0	978-992 bp	1.0	77.0
rpl2-trnI-CAU_p4	AACGACGCTGTCCATAGATC	GAATGGTTAAAGCGCCCAAC	50.0	50.0	57.5	58.0	980-994 bp	1.0	77.0
rpl2-trnI-CAU_p5	CAACGACGCTGTCCATAGAT	GCTAAGCATCCATGGCTGAA	50.0	50.0	57.8	58.3	998-1012 bp	1.0	76.8