Hotspot

psaJ-rpl33

LSC · 67564-67988 · 425 bp

Back to Krascheninnikovia

Pi 0.00476
MarkerSeek score 46.40
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
425
variable_sites
2
pi
0.00476
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 GATGCGTTGACATTCCCTTT CCACTCTTTCGAACACAAGC 45.0 50.0 56.7 57.3 521-526 bp 1.0 65.3
psaJ-rpl33_p2 TGCGTTGACATTCCCTTTTT CCACTCTTTCGAACACAAGC 40.0 50.0 56.4 57.3 519-524 bp 1.0 63.3
psaJ-rpl33_p3 GATGCGTTGACATTCCCTTTT CCACTCTTTCGAACACAAGC 42.9 50.0 57.4 57.3 521-526 bp 1.0 63.1
psaJ-rpl33_p4 GATGCGTTGACATTCCCTTT ACTCTTTCGAACACAAGCAGT 45.0 42.9 56.7 58.1 519-524 bp 1.0 61.9
psaJ-rpl33_p5 ATGCGTTGACATTCCCTTTT CCACTCTTTCGAACACAAGC 40.0 50.0 56.2 57.3 520-525 bp 1.0 61.4