Hotspot

rpoB-trnC-GCA

LSC · 26954-28093 · 1140 bp

Back to Krascheninnikovia

Pi 0.000883

MarkerSeek score 59.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1140
variable_sites: 1
pi: 0.000883
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ACATTCCCTCGTTTCGATCC	GGCGTCGATAAGTGAGTTGT	50.0	50.0	57.7	58.0	1279-1287 bp	1.0	78.0
rpoB-trnC-GCA_p2	GCCCTGATCAATGAACCTCC	GGCGTCGATAAGTGAGTTGT	55.0	50.0	58.3	58.0	1341-1349 bp	1.0	78.0
rpoB-trnC-GCA_p3	ACATTCCCTCGTTTCGATCC	CGGATTTGAACTGGGGAACA	50.0	50.0	57.7	58.1	1221-1229 bp	1.0	77.3
rpoB-trnC-GCA_p4	GCCCTGATCAATGAACCTCC	CGGATTTGAACTGGGGAACA	55.0	50.0	58.3	58.1	1283-1291 bp	1.0	77.3
rpoB-trnC-GCA_p5	CATTCCCTCGTTTCGATCCC	GGCGTCGATAAGTGAGTTGT	55.0	50.0	58.4	58.0	1278-1286 bp	1.0	77.2