Hotspot

atpH-atpI

LSC · 13946-14722 · 777 bp

Back to Krascheninnikovia

Pi 0.00129

MarkerSeek score 59.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 777
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.0	57.9	864 bp	1.0	78.7
atpH-atpI_p2	GCCAACTCCAGGTCCAATAG	TTTTGCAACTTTAGCCGCAG	55.0	45.0	58.0	57.9	913 bp	1.0	78.7
atpH-atpI_p3	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	57.9	57.9	856 bp	1.0	78.5
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	57.9	57.9	880 bp	1.0	77.8
atpH-atpI_p5	AAGCGGCAGAAATCAATGGA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.2	57.9	857 bp	1.0	77.7