Hotspot

rps16

LSC · 7217-9038 · 1822 bp

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Pi 0.0174

MarkerSeek score 61.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1822
variable_sites: 30
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGTAATCCACACCCTCTTTT	ACTGGTTAAAAAGAAAGGAATGT	40.0	30.4	53.8	54.6	1843-1886 bp	1.0	45.3
rps16_p2	AAAAGTAATCCACACCCTCT	TGTATCAAATGAACCAATGACT	40.0	31.8	53.8	53.7	1899-1942 bp	1.0	45.2
rps16_p3	AAAGTAATCCACACCCTCTT	TGTATCAAATGAACCAATGACT	40.0	31.8	53.8	53.7	1898-1941 bp	1.0	45.2
rps16_p4	AAGTAATCCACACCCTCTTT	TGTATCAAATGAACCAATGACT	40.0	31.8	53.8	53.7	1897-1940 bp	1.0	45.2
rps16_p5	AGTAATCCACACCCTCTTTT	TGTATCAAATGAACCAATGACT	40.0	31.8	53.8	53.7	1896-1939 bp	1.0	45.2