Hotspot

ndhF-rpl32

SSC · 128919-130581 · 1663 bp

Back to Krameria

Pi 0.0276

MarkerSeek score 76.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1663
variable_sites: 45
pi: 0.0276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAACAGGAAGTGGAATAAAAGG	TCAATGCTGCCCAATATCCC	43.5	50.0	57.4	58.0	1807-1929 bp	1.0	54.2
ndhF-rpl32_p2	GGAACAGGAAGTGGAATAAAAGG	CAATGCTGCCCAATATCCCT	43.5	50.0	57.4	58.0	1806-1928 bp	1.0	54.1
ndhF-rpl32_p3	GGAACAGGAAGTGGAATAAAAGG	AAAAGCTTTCAATGCTGCCC	43.5	45.0	57.4	57.8	1815-1937 bp	1.0	52.8
ndhF-rpl32_p4	GGAACAGGAAGTGGAATAAAAGG	TGCTGCCCAATATCCCTTTT	43.5	45.0	57.4	57.5	1803-1925 bp	1.0	49.8
ndhF-rpl32_p5	AAAAGAGAAGTCCCATTCCT	TCAATGCTGCCCAATATCCC	40.0	50.0	53.8	58.0	1836-1958 bp	1.0	49.7