Hotspot

accD-psaI

LSC · 71446-72316 · 871 bp

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Pi 0.0134

MarkerSeek score 64.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 871
variable_sites: 11
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAGGGCGTTTTGAGTGAAT	GCCGGAAAGACTAAGCCTAC	40.0	55.0	56.1	58.1	948-994 bp	1.0	69.5
accD-psaI_p2	AAGGGCGTTTTGAGTGAATT	GCCGGAAAGACTAAGCCTAC	40.0	55.0	56.1	58.1	947-993 bp	1.0	69.5
accD-psaI_p3	AAAGGGCGTTTTGAGTGAAT	AAGAAGCCATTGCAATTGCC	40.0	45.0	56.1	57.9	965-1011 bp	1.0	69.1
accD-psaI_p4	AAGGGCGTTTTGAGTGAATT	AAGAAGCCATTGCAATTGCC	40.0	45.0	56.1	57.9	964-1010 bp	1.0	69.1
accD-psaI_p5	AAGGGCGTTTTGAGTGAATT	TGCAATTGCCGGAAAGACTA	40.0	45.0	56.1	57.8	954-1000 bp	1.0	68.4