Hotspot

atpH-atpI

LSC · 21532-22390 · 859 bp

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Pi 0.00815

MarkerSeek score 63.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 859
variable_sites: 7
pi: 0.00815
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	946 bp	1.0	79.2
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	962 bp	1.0	78.3
atpH-atpI_p3	AACGGAAGCGGCAGAAATAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	944 bp	1.0	78.1
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	947 bp	1.0	78.1
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.8	57.9	961 bp	1.0	77.8