Hotspot

atpF-atpH

LSC · 20831-21285 · 455 bp

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Pi 0.0117

MarkerSeek score 67.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 5
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TATTCAACCCGAAACTCCCG	CCGAGGCAGAGGGAAAAATA	50.0	50.0	57.6	57.3	643-644 bp	1.0	75.1
atpF-atpH_p2	TATTCAACCCGAAACTCCCG	GGACTGGTTGTAGCATTAGCA	50.0	47.6	57.6	58.0	572-573 bp	1.0	73.1
atpF-atpH_p3	TATTCAACCCGAAACTCCCG	TGGACTGGTTGTAGCATTAGC	50.0	47.6	57.6	58.0	573-574 bp	1.0	73.1
atpF-atpH_p4	TATTCAACCCGAAACTCCCG	CCGAGGCAGAGGGAAAAATAA	50.0	47.6	57.6	58.0	643-644 bp	1.0	72.7
atpF-atpH_p5	CGGATGCCAAATGACCCAAA	CCGAGGCAGAGGGAAAAATA	50.0	50.0	59.1	57.3	622-623 bp	1.0	69.5