Hotspot

trnR-UCU-atpA

LSC · 17148-17899 · 752 bp

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Pi 0.0281

MarkerSeek score 77.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 20
pi: 0.0281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CCGAGGAAGCAGAAACACTT	47.6	50.0	57.3	58.1	814-851 bp	1.0	71.2
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	CAGGAACAGATGGAACGCTT	47.6	50.0	57.3	58.2	779-816 bp	1.0	70.9
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	TTCAGGAACAGATGGAACGC	47.6	50.0	57.3	58.2	781-818 bp	1.0	70.8
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	TACCGAGGAAGCAGAAACAC	47.6	50.0	57.3	57.5	816-853 bp	1.0	68.5
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	CCGAGGAAGCAGAAACACTT	47.6	50.0	56.9	58.1	849-886 bp	1.0	68.1