Hotspot

FNM16_pgt005-trnR-UCU

LSC · 16346-17075 · 730 bp

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Pi 0.0404

MarkerSeek score 82.50

Primer available yes

Label FNM16_pgt005-trnR-UCU

Feature Metadata

label_name: FNM16_pgt005-trnR-UCU
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 28
pi: 0.0404
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
FNM16_pgt005-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	804-810 bp	1.0	69.8
FNM16_pgt005-trnR-UCU_p2	ACCCTTAGCCTTCCAAGCTA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	810-816 bp	1.0	69.7
FNM16_pgt005-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	810-816 bp	1.0	69.1
FNM16_pgt005-trnR-UCU_p4	ACCCTTAGCCTTCCAAGCTA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	816-822 bp	1.0	69.0
FNM16_pgt005-trnR-UCU_p5	CTAACGATGCGGGTTCGATT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.4	56.9	793-799 bp	1.0	66.7