Hotspot

trnS-GCU-FNM16_pgt005

LSC · 14579-15536 · 958 bp

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Pi 0.0315
MarkerSeek score 73.70
Primer available yes
Label trnS-GCU-FNM16_pgt005

Feature Metadata

label_name
trnS-GCU-FNM16_pgt005
feature_type
igs
region
LSC
length_bp
958
variable_sites
30
pi
0.0315
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-FNM16_pgt005_p1 CAATCCGACGCTTTAGTCCA TCAAGAAAACTGATGTACCCCT 50.0 40.9 58.0 57.0 1072-1485 bp 1.0 58.2
trnS-GCU-FNM16_pgt005_p2 AATCCGACGCTTTAGTCCAC TCAAGAAAACTGATGTACCCCT 50.0 40.9 58.0 57.0 1071-1484 bp 1.0 58.2
trnS-GCU-FNM16_pgt005_p3 AAAGAACCGACGTCAATGGT TCAAGAAAACTGATGTACCCCT 45.0 40.9 57.8 57.0 1148-1561 bp 1.0 56.1
trnS-GCU-FNM16_pgt005_p4 CAATCCGACGCTTTAGTCCA TCAAGAAAACTGATGTACCCCTT 50.0 39.1 58.0 57.7 1072-1485 bp 1.0 55.6
trnS-GCU-FNM16_pgt005_p5 AATCCGACGCTTTAGTCCAC TCAAGAAAACTGATGTACCCCTT 50.0 39.1 58.0 57.7 1071-1484 bp 1.0 55.6