Hotspot

trnQ-UUG-psbK

LSC · 10200-13552 · 3353 bp

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Pi 0.016

MarkerSeek score 47.10

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 3353
variable_sites: 36
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	TGATTCTATTCATTATTCGCGA	ATTTGGAATGAAAGATTTTGGA	31.8	27.3	53.6	52.5	556-1593 bp	1.0	44.3
trnQ-UUG-psbK_p2	TGATTCTATTCATTATTCGCGA	TTTGGAATGAAAGATTTTGGAT	31.8	27.3	53.6	52.5	555-1592 bp	1.0	44.3
trnQ-UUG-psbK_p3	TCTATTCATTATTCGCGATTTCA	ATTTGGAATGAAAGATTTTGGA	30.4	27.3	54.4	52.5	552-1589 bp	1.0	44.3
trnQ-UUG-psbK_p4	TCTATTCATTATTCGCGATTTCA	TTTGGAATGAAAGATTTTGGAT	30.4	27.3	54.4	52.5	551-1588 bp	1.0	44.3
trnQ-UUG-psbK_p5	TTGATTCTATTCATTATTCGCGA	TTTGGAATGAAAGATTTTGGAT	30.4	27.3	54.4	52.5	556-1593 bp	1.0	44.3