Hotspot

rps16-trnQ-UUG

LSC · 9039-10127 · 1089 bp

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Pi 0.0393
MarkerSeek score 77.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1089
variable_sites
40
pi
0.0393
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCACAACGGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1135-1183 bp 1.0 85.2
rps16-trnQ-UUG_p2 ACACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.7 58.1 1109-1157 bp 1.0 83.7
rps16-trnQ-UUG_p3 TCACACGTTGCTTTCTACCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.4 58.1 1111-1159 bp 1.0 81.4
rps16-trnQ-UUG_p4 CACACGTTGCTTTCTACCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.3 58.1 1110-1158 bp 1.0 80.9
rps16-trnQ-UUG_p5 TGTCCTTCAAGTCACACGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.3 58.1 1122-1170 bp 1.0 80.7