Hotspot

FNM16_pgt002

LSC · 3889-3923 · 35 bp

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Pi 0.0588

MarkerSeek score 72.20

Primer available yes

Label FNM16_pgt002

Feature Metadata

label_name: FNM16_pgt002
feature_type: tRNA
region: LSC
length_bp: 35
variable_sites: 2
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
FNM16_pgt002_p1	GGACTCCCAAGCACACAAAT	TTATCGACCGATTTGTGCGT	50.0	45.0	58.4	58.0	1329-1335 bp	1.0	78.9
FNM16_pgt002_p2	GGACTCCCAAGCACACAAAT	ACAAAAGTACTGTACGCGCT	50.0	45.0	58.4	57.9	1200-1206 bp	1.0	77.9
FNM16_pgt002_p3	GGACTCCCAAGCACACAAAT	CACAAAAGTACTGTACGCGC	50.0	50.0	58.4	57.8	1201-1207 bp	1.0	77.4
FNM16_pgt002_p4	GGACTCCCAAGCACACAAAT	ATCGACCGATTTGTGCGTAT	50.0	45.0	58.4	57.8	1327-1333 bp	1.0	77.1
FNM16_pgt002_p5	GGACTCCCAAGCACACAAAT	CGGTTTGGGGAGGGATTTTT	50.0	50.0	58.4	58.4	758-764 bp	1.0	76.7