Hotspot

ycf1

SSC · 126927-132212 · 5286 bp

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Pi 0.0112

MarkerSeek score 47.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5286
variable_sites: 59
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGGTTTCACTCAATCCAGA	CGGAAGTGGAATTCCCCTTT	45.0	50.0	56.4	57.8	268 bp	1.0	66.9
ycf1_p2	TTGGGTTTCACTCAATCCAGA	CGGAAGTGGAATTCCCCTTT	42.9	50.0	57.1	57.8	269 bp	1.0	65.1
ycf1_p3	TGGGTTTCACTCAATCCAGA	CTTTTCTCGTTACCCCCGAA	45.0	50.0	56.4	57.5	252 bp	1.0	65.1
ycf1_p4	TGGGTTTCACTCAATCCAGAT	CGGAAGTGGAATTCCCCTTT	42.9	50.0	56.9	57.8	268 bp	1.0	63.3
ycf1_p5	TTGGGTTTCACTCAATCCAGA	CTTTTCTCGTTACCCCCGAA	42.9	50.0	57.1	57.5	253 bp	1.0	63.2