Hotspot

rpl32-trnL-UAG

SSC · 115792-116767 · 976 bp

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Pi 0.0219

MarkerSeek score 62.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 976
variable_sites: 21
pi: 0.0219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCGCAATCTATTTCGACAGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.3	57.9	1049-1060 bp	1.0	76.6
rpl32-trnL-UAG_p2	GCGCAATCTATTTCGACAGG	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.3	57.8	1042-1053 bp	1.0	76.1
rpl32-trnL-UAG_p3	GCGCAATCTATTTCGACAGG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.3	57.8	1048-1059 bp	1.0	76.0
rpl32-trnL-UAG_p4	GCGCAATCTATTTCGACAGG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.3	57.8	1047-1058 bp	1.0	76.0
rpl32-trnL-UAG_p5	GCGCAATCTATTTCGACAGG	GAGCAGCGTGTCTACCAATT	50.0	50.0	57.3	58.3	1043-1054 bp	1.0	75.4