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rps8-rpl14

LSC · 83332-83765 · 434 bp

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Pi 0.0211

MarkerSeek score 68.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 434
variable_sites: 9
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TACGTCAGCGATAGTGTCCT	CGCAATCCCCCGAGAATTAA	50.0	50.0	58.0	58.0	531-545 bp	1.0	81.8
rps8-rpl14_p2	AGCGATAGTGTCCTTACCCA	CGCAATCCCCCGAGAATTAA	50.0	50.0	57.8	58.0	525-539 bp	1.0	80.9
rps8-rpl14_p3	TACGTCAGCGATAGTGTCCT	AGAATTTTTGGCGCAATCCC	50.0	45.0	58.0	57.6	542-556 bp	1.0	79.0
rps8-rpl14_p4	AGCGATAGTGTCCTTACCCA	AGAATTTTTGGCGCAATCCC	50.0	45.0	57.8	57.6	536-550 bp	1.0	78.0
rps8-rpl14_p5	CAGCGATAGTGTCCTTACCC	CGCAATCCCCCGAGAATTAA	55.0	50.0	57.5	58.0	526-540 bp	1.0	78.0