Hotspot

psbK-trnQ-UUG

LSC · 57115-57513 · 399 bp

Back to Kotschya

Pi 0.015

MarkerSeek score 54.10

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 6
pi: 0.015
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	AGGCTTCAGGTAATTTCGCC	ATATCCTCTGGGACGGAAGG	50.0	55.0	58.2	58.0	566-570 bp	1.0	78.7
psbK-trnQ-UUG_p2	GGCTTCAGGTAATTTCGCCA	ATATCCTCTGGGACGGAAGG	50.0	55.0	58.5	58.0	565-569 bp	1.0	76.4
psbK-trnQ-UUG_p3	AAAGGGCGGAGTGAATACAA	ATATCCTCTGGGACGGAAGG	45.0	55.0	57.1	58.0	527-531 bp	1.0	73.8
psbK-trnQ-UUG_p4	AAGGGCGGAGTGAATACAAA	ATATCCTCTGGGACGGAAGG	45.0	55.0	57.1	58.0	526-530 bp	1.0	73.8
psbK-trnQ-UUG_p5	AGGCTTCAGGTAATTTCGCC	GACCAAAACCCGTTGCCTTA	50.0	50.0	58.2	58.7	525-529 bp	1.0	73.5