Hotspot

rpoB-rpoC1

LSC · 39790-39815 · 26 bp

Back to Kotschya

Pi 0.0385

MarkerSeek score 74.60

Primer available yes

Label rpoB-rpoC1

Feature Metadata

label_name: rpoB-rpoC1
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 1
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-rpoC1_p1	TTGCTCGTTCGAGAACTACG	GGTTTTGTTACCTCGCCAAC	50.0	50.0	58.0	57.6	238 bp	1.0	76.7
rpoB-rpoC1_p2	TTGCTCGTTCGAGAACTACG	GGGTTTTGTTACCTCGCCAA	50.0	50.0	58.0	58.7	239 bp	1.0	74.6
rpoB-rpoC1_p3	TTCGATTGCTCGTTCGAGAA	GGTTTTGTTACCTCGCCAAC	45.0	50.0	57.7	57.6	243 bp	1.0	74.3
rpoB-rpoC1_p4	TTTCGATTGCTCGTTCGAGA	GGTTTTGTTACCTCGCCAAC	45.0	50.0	57.7	57.6	244 bp	1.0	74.3
rpoB-rpoC1_p5	CGATTGCTCGTTCGAGAACT	GGTTTTGTTACCTCGCCAAC	50.0	50.0	58.4	57.6	241 bp	1.0	74.0