Hotspot

trnK-UUU

LSC · 1628-1662 · 35 bp

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Pi 0.0381
MarkerSeek score 69.40
Primer available yes
Label trnK-UUU

Feature Metadata

label_name
trnK-UUU
feature_type
tRNA
region
LSC
length_bp
35
variable_sites
2
pi
0.0381
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU_p1 TGCTAGTGTCAACCAATATCGA TGGGGAGGGGTGTTTACTTA 40.9 50.0 57.3 57.6 189 bp 1.0 55.5
trnK-UUU_p2 TGCTAGTGTCAACCAATATCGA TACATAGGGAAAGCCGTGTG 40.9 50.0 57.3 57.3 232 bp 1.0 53.4
trnK-UUU_p3 TGCTAGTGTCAACCAATATCG TGGGGAGGGGTGTTTACTTA 42.9 50.0 55.8 57.6 189 bp 1.0 51.7
trnK-UUU_p4 TGCTAGTGTCAACCAATATCGA CTGGGGAGGGGTGTTTACTT 40.9 55.0 57.3 58.9 190 bp 1.0 51.6
trnK-UUU_p5 TGCTAGTGTCAACCAATATCGAT TGGGGAGGGGTGTTTACTTA 39.1 50.0 57.7 57.6 189 bp 1.0 51.1