Hotspot

psbE-petL

LSC · 74854-75902 · 1049 bp

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Pi 0.00953
MarkerSeek score 63.20
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1049
variable_sites
15
pi
0.00953
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GTCGTATCTTGCTCAAGCCA 50.0 50.0 57.9 58.0 1241-1382 bp 1.0 80.4
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC GTCGTATCTTGCTCAAGCCA 50.0 50.0 57.8 58.0 1164-1305 bp 1.0 79.7
psbE-petL_p3 ACGAATAACCAACCCGCAAT GTCGTATCTTGCTCAAGCCA 45.0 50.0 58.2 58.0 1250-1391 bp 1.0 79.5
psbE-petL_p4 TGCTGACGAATAACCAACCC GTCGTATCTTGCTCAAGCCA 50.0 50.0 58.2 58.0 1255-1396 bp 1.0 79.4
psbE-petL_p5 CGAATAACCAACCCGCAATG GTCGTATCTTGCTCAAGCCA 50.0 50.0 57.8 58.0 1249-1390 bp 1.0 79.3