Hotspot

trnS-GCU-trnG-UCC

LSC · 8317-18480 · 10164 bp

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Pi 0.03
MarkerSeek score 49.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
10164
variable_sites
116
pi
0.03
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.34
markerseek_score
49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 GCGATCGCATCTCGTTAGAT TTGTTTTGCTCGGTTCTCGA 50.0 45.0 58.0 58.1 1738 bp 0.333333 52.6
trnS-GCU-trnG-UCC_p2 TTTCTCATCTCATCAGCCGC TTGTTTTGCTCGGTTCTCGA 50.0 45.0 58.1 58.1 2137 bp 0.333333 52.4
trnS-GCU-trnG-UCC_p3 AATGTTTCGTTCCGTCGGAT TTGTTTTGCTCGGTTCTCGA 45.0 45.0 57.9 58.1 1648 bp 0.333333 52.2
trnS-GCU-trnG-UCC_p4 TGGTCATTCCAAAGAGAGCG TTGTTTTGCTCGGTTCTCGA 50.0 45.0 57.9 58.1 2168 bp 0.333333 52.1
trnS-GCU-trnG-UCC_p5 GGTCATTCCAAAGAGAGCGT TTGTTTTGCTCGGTTCTCGA 50.0 45.0 57.9 58.1 2167 bp 0.333333 52.1