Hotspot

ndhA

SSC · 124339-126494 · 2156 bp

Back to Koenigia

Pi 0.0605

MarkerSeek score 79.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2156
variable_sites: 330
pi: 0.0605
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AGCGTTCTGATGCGATCAAT	GGCTTCCGGAATAGAATGGG	45.0	55.0	58.1	58.1	2888-2920 bp	0.9	85.6
ndhA_p2	AGCGTTCTGATGCGATCAAT	GGGCTTCCGGAATAGAATGG	45.0	55.0	58.1	58.1	2889-2921 bp	0.9	85.6
ndhA_p3	AGCGTTCTGATGCGATCAAT	GGGTTGTCAGGACCAATGTT	45.0	50.0	58.1	58.0	2918-2943 bp	0.6	71.2
ndhA_p4	AGCGTTCTGATGCGATCAAT	AAATTGGGGGTTGTCAGGAC	45.0	50.0	58.1	58.0	2925-2950 bp	0.6	71.2
ndhA_p5	AGCGTTCTGATGCGATCAAT	TTTGGAACGAGTTGAAGGGG	45.0	50.0	58.1	58.0	2976-2998 bp	0.4	62.2