Hotspot

atpF

LSC · 11422-12755 · 1334 bp

Back to Koenigia

Pi 0.0447

MarkerSeek score 78.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1334
variable_sites: 157
pi: 0.0447
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TGACTACCTGTGCTACCGAA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.1	58.0	2649-2706 bp	0.7	76.6
atpF_p2	CTGTGCTACCGAAGATGCTT	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	2535-2699 bp	0.6	71.8
atpF_p3	CTGTGCTACCGAAGATGCTT	AGGCCAAGCTGTAGAAGGTA	50.0	50.0	58.0	58.0	2452-2616 bp	0.6	71.8
atpF_p4	ACCAGTCTGTCTGTCTCCAA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	57.9	58.0	2436-2600 bp	0.6	71.2
atpF_p5	TGACTACCTGTGCTACCGAA	AGGCCAAGCTGTAGAAGGTA	50.0	50.0	58.1	58.0	2577-2623 bp	0.5	67.4