Hotspot

trnN-GUU-trnR-ACG

IRa · 134607-135216 · 610 bp

Back to Koenigia

Pi 0.0376

MarkerSeek score 81.90

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 610
variable_sites: 56
pi: 0.0376
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	732-754 bp	1.0	87.1
trnN-GUU-trnR-ACG_p2	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	696-718 bp	1.0	86.4
trnN-GUU-trnR-ACG_p3	CCGTTGTTCCTCAGTAGCTC	GGATACTCCCGGGAACAGAT	55.0	55.0	58.0	58.3	781-803 bp	1.0	86.3
trnN-GUU-trnR-ACG_p4	ATCAAATCCCCCGTTGTTCC	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	742-764 bp	1.0	85.8
trnN-GUU-trnR-ACG_p5	GGATCAAATCCCCCGTTGTT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	744-766 bp	1.0	85.8