Hotspot

ndhF-rpl32

SSC · 116890-117874 · 985 bp

Back to Koenigia

Pi 0.0723

MarkerSeek score 70.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 985
variable_sites: 147
pi: 0.0723
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.60
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCCACATACGACGAAGATT	GTACGCTTTTTGGGAACTGC	50.0	50.0	58.1	58.0	452-1130 bp	1.0	91.1
ndhF-rpl32_p2	AGGAAAAGCCCACATACGAC	GTACGCTTTTTGGGAACTGC	50.0	50.0	57.9	58.0	459-1137 bp	1.0	90.8
ndhF-rpl32_p3	AAAAGCCCACATACGACGAA	GTACGCTTTTTGGGAACTGC	45.0	50.0	57.8	58.0	456-1134 bp	1.0	90.2
ndhF-rpl32_p4	GGGCGTGTAAACCAAAAACC	GTACGCTTTTTGGGAACTGC	50.0	50.0	58.1	58.0	836-1514 bp	0.9	86.5
ndhF-rpl32_p5	TTCTAAACTGCCCGTTAGCC	GTACGCTTTTTGGGAACTGC	50.0	50.0	57.9	58.0	942-1620 bp	0.6	72.5