Hotspot

trnR-ACG-trnN-GUU

IRb · 107558-108167 · 610 bp

Back to Koenigia

Pi 0.0375

MarkerSeek score 81.90

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 610
variable_sites: 56
pi: 0.0375
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	732-754 bp	1.0	87.1
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	696-718 bp	1.0	86.4
trnR-ACG-trnN-GUU_p3	GGATACTCCCGGGAACAGAT	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	58.3	58.0	781-803 bp	1.0	86.3
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	ATCAAATCCCCCGTTGTTCC	55.0	50.0	57.8	58.2	742-764 bp	1.0	85.8
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	GGATCAAATCCCCCGTTGTT	55.0	50.0	57.8	58.2	744-766 bp	1.0	85.8