Hotspot

psaJ-rpl33

LSC · 66678-67133 · 456 bp

Back to Koenigia

Pi 0.0829

MarkerSeek score 77.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 456
variable_sites: 88
pi: 0.0829
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACCCGTGACATTTTGTACCC	ATTCCAATCGACTGGGTGTG	50.0	50.0	58.1	57.9	1042-1152 bp	1.0	90.3
psaJ-rpl33_p2	CCCGTGACATTTTGTACCCA	ATTCCAATCGACTGGGTGTG	50.0	50.0	58.1	57.9	1041-1151 bp	1.0	90.3
psaJ-rpl33_p3	TTTTCCCGGATGCGTTAACA	ATTCCAATCGACTGGGTGTG	45.0	50.0	58.1	57.9	487-639 bp	1.0	90.2
psaJ-rpl33_p4	GGATGCGTTAACATTCCCCT	ATTCCAATCGACTGGGTGTG	50.0	50.0	57.9	57.9	605-632 bp	0.4	62.7
psaJ-rpl33_p5	GGATGCGTTAACATTCCCCT	GGGTGTGTTGTGTCGATTCT	50.0	50.0	57.9	58.1	592-619 bp	0.4	62.7