Hotspot

ycf3-trnS-GGA

LSC · 44491-45366 · 876 bp

Back to Koenigia

Pi 0.0645

MarkerSeek score 82.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 876
variable_sites: 144
pi: 0.0645
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	ACTTCACCTTCAACAAGCGT	GGAGCCCGCCAATTAGTTAA	45.0	50.0	58.0	58.0	1584-1740 bp	1.0	91.3
ycf3-trnS-GGA_p2	TTCGCCTGAAGTTGTTGGAA	TGAGCCCCCTATTCTCCAAA	45.0	50.0	58.0	58.0	1134-1291 bp	0.6	73.1
ycf3-trnS-GGA_p3	TTTCGCCTGAAGTTGTTGGA	TGAGCCCCCTATTCTCCAAA	45.0	50.0	58.0	58.0	1135-1292 bp	0.6	73.1
ycf3-trnS-GGA_p4	ACTTCACCTTCAACAAGCGT	TGAGCCCCCTATTCTCCAAA	45.0	50.0	58.0	58.0	1262-1419 bp	0.6	72.8
ycf3-trnS-GGA_p5	ACTTCACCTTCAACAAGCGT	TGTTGGTGAGTTGGGTGAAG	45.0	50.0	58.0	57.9	1126-1210 bp	0.4	61.1