Hotspot

trnE-UUC-trnT-GGU

LSC · 30746-31597 · 852 bp

Back to Koenigia

Pi 0.109

MarkerSeek score 84.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 852
variable_sites: 226
pi: 0.109
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	922-1083 bp	1.0	89.8
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	901-1062 bp	1.0	89.7
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	901-1062 bp	1.0	89.4
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	880-1041 bp	1.0	89.2
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.3	58.1	898-1059 bp	1.0	89.2