Hotspot

ycf2

IRa · 153716-160621 · 6906 bp

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Pi 0.466

MarkerSeek score 88.40

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 6906
variable_sites: 2609
pi: 0.466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 88.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GTGCTACAAGATCTCGTGCA	CCGCTGATGGGGTTAAATCA	50.0	50.0	58.0	57.9	1302 bp	0.5	59.6
ycf2_p2	AGGCTTGGTATCCAGGAACT	CCGCTGATGGGGTTAAATCA	50.0	50.0	58.0	57.9	710 bp	0.5	59.4
ycf2_p3	AGATCTCGTGCATTGGAACC	CCGCTGATGGGGTTAAATCA	50.0	50.0	58.0	57.9	1294 bp	0.5	59.4
ycf2_p4	GTGCTACAAGATCTCGTGCA	TTCTCTTTGTTTCCCGAGCC	50.0	50.0	58.0	58.1	1515 bp	0.5	59.1
ycf2_p5	CAGCTCCGTATCAAGGTCAC	CCGCTGATGGGGTTAAATCA	55.0	50.0	58.1	57.9	854 bp	0.5	59.0