Hotspot

petA-psbJ

LSC · 68052-68922 · 871 bp

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Pi 0.00366

MarkerSeek score 65.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 871
variable_sites: 3
pi: 0.00366
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCTGGATTGGGTTCGTCG	50.0	50.0	58.0	58.0	976-1016 bp	1.0	83.7
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	GGATTGGGTTCGTCGTTGTA	50.0	50.0	58.0	57.9	971-1011 bp	1.0	83.3
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	ATTCTGGATTGGGTTCGTCG	50.0	50.0	58.2	58.0	973-1013 bp	1.0	82.6
petA-psbJ_p4	TTTGGCATCCGTTGTTTTGG	ATTCTGGATTGGGTTCGTCG	45.0	50.0	57.8	58.0	945-985 bp	1.0	82.2
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	GGATTGGGTTCGTCGTTGTA	50.0	50.0	58.2	57.9	968-1008 bp	1.0	82.1