Hotspot

trnF-GAA-ndhJ

LSC · 52868-53651 · 784 bp

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Pi 0.0116

MarkerSeek score 66.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 9
pi: 0.0116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TCCACGGCTGAAACGTATTT	50.0	45.0	58.1	57.8	921-1023 bp	1.0	79.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	893-995 bp	1.0	79.1
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CATCCACGGCTGAAACGTAT	50.0	50.0	58.1	58.3	923-1025 bp	1.0	78.2
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATCCACGGCTGAAACGTATT	50.0	45.0	58.1	57.6	922-1024 bp	1.0	77.8
trnF-GAA-ndhJ_p5	TGTGTCAGTAATGGTCGGGA	TCCACGGCTGAAACGTATTT	50.0	45.0	58.4	57.8	973-1075 bp	1.0	77.0