Hotspot

trnR-UCU-atpA

LSC · 11158-11494 · 337 bp

Back to Koelreuteria

Pi 0.0245

MarkerSeek score 70.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 337
variable_sites: 8
pi: 0.0245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGCAAAGCGTCCATTGTCTA	AAGACATTCACCGAGGAAGC	45.0	50.0	57.8	57.9	502 bp	1.0	80.6
trnR-UCU-atpA_p2	AATTCGGAATGCAAAGCGTC	AAGACATTCACCGAGGAAGC	45.0	50.0	57.7	57.9	511 bp	1.0	79.9
trnR-UCU-atpA_p3	TGCAAAGCGTCCATTGTCTA	TCAGGAACAAATGGAACGCT	45.0	45.0	57.8	57.7	458 bp	1.0	79.6
trnR-UCU-atpA_p4	AATGCAAAGCGTCCATTGTC	AAGACATTCACCGAGGAAGC	45.0	50.0	57.6	57.9	504 bp	1.0	79.2
trnR-UCU-atpA_p5	GAATGCAAAGCGTCCATTGT	AAGACATTCACCGAGGAAGC	45.0	50.0	57.6	57.9	505 bp	1.0	79.2