Hotspot

trnS-GCU-trnG-UCC

LSC · 9191-10099 · 909 bp

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Pi 0.0108
MarkerSeek score 68.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
909
variable_sites
9
pi
0.0108
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CCGACGCTTTAATCCACTCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 903-974 bp 1.0 74.8
trnS-GCU-trnG-UCC_p2 CCGACGCTTTAATCCACTCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 903-974 bp 1.0 74.8
trnS-GCU-trnG-UCC_p3 GCTCGTACAACGGATTAGCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.1 58.2 925-996 bp 1.0 74.2
trnS-GCU-trnG-UCC_p4 GCTCGTACAACGGATTAGCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.1 56.8 925-996 bp 1.0 74.1
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.2 58.2 961-1032 bp 1.0 73.6