Hotspot

petB

LSC · 70959-72371 · 1413 bp

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Pi 0.00189

MarkerSeek score 60.10

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1413
variable_sites: 5
pi: 0.00189
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TAGTTCGACCGCGGAATTTT	ACCCATGTTTGTAGCAATGA	45.0	40.0	57.9	55.2	1536-1543 bp	1.0	57.4
petB_p2	ATTATGGTAGTTCGACCGCG	ACCCATGTTTGTAGCAATGA	50.0	40.0	57.9	55.2	1543-1550 bp	1.0	57.1
petB_p3	TAGTTCGACCGCGGAATTTT	AACCCATGTTTGTAGCAATGA	45.0	38.1	57.9	56.0	1537-1544 bp	1.0	56.0
petB_p4	TAGTTCGACCGCGGAATTTT	ACCCATGTTTGTAGCAATGAA	45.0	38.1	57.9	56.0	1536-1543 bp	1.0	56.0
petB_p5	ATTATGGTAGTTCGACCGCG	ACCCATGTTTGTAGCAATGAA	50.0	38.1	57.9	56.0	1543-1550 bp	1.0	55.6