Hotspot

ccsA

SSC · 105938-106882 · 945 bp

Back to Koeleria

Pi 0.00106

MarkerSeek score 36.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 945
variable_sites: 2
pi: 0.00106
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTCAAGCATCTCGGTTCGAA	TGAGTGGTTTTGTCGCAGAA	50.0	45.0	58.0	58.0	1540-1561 bp	1.0	80.4
ccsA_p2	CTCAAGCATCTCGGTTCGAA	TCTTGCCCTACCAGGAATGA	50.0	50.0	58.0	58.0	1557-1578 bp	1.0	80.4
ccsA_p3	CTCAAGCATCTCGGTTCGAA	TTCTCTTGCCCTACCAGGAA	50.0	50.0	58.0	58.0	1560-1581 bp	1.0	80.4
ccsA_p4	CTCAAGCATCTCGGTTCGAA	ATCTCGAAGAACTTGGGGGA	50.0	50.0	58.0	58.0	1633-1654 bp	1.0	80.3
ccsA_p5	AAGCATCTCGGTTCGAATCC	TGAGTGGTTTTGTCGCAGAA	50.0	45.0	58.1	58.0	1537-1558 bp	1.0	80.0