Hotspot

atpB

LSC · 51834-53330 · 1497 bp

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Pi 0.00134

MarkerSeek score 59.70

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 4
pi: 0.00134
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	GTGGTTTGGTAATACGCCGA	TTTTTGGATTTGATTTGGCA	50.0	30.0	58.3	52.3	1683-1684 bp	1.0	40.2
atpB_p2	GTGGTTTGGTAATACGCCGA	GGATTTGATTTGGCATTATTCAACA	50.0	32.0	58.3	57.2	1678-1679 bp	1.0	40.2
atpB_p3	GTGGTTTGGTAATACGCCGA	TGGATTTGATTTGGCATTATTCAAC	50.0	32.0	58.3	57.2	1679-1680 bp	1.0	40.2
atpB_p4	GTGGTTTGGTAATACGCCGA	TGGATTTGATTTGGCATTATTCA	50.0	30.4	58.3	55.1	1679-1680 bp	1.0	40.2
atpB_p5	GTGGTTTGGTAATACGCCGA	TGATTTGGCATTATTCAACAAGA	50.0	30.4	58.3	55.1	1673-1674 bp	1.0	40.2